Counting Bacteria

Counting viruses and bacteria in photosynthetic microbial

A method for counting acid-fast bacteria.

A reliable, convenient, and accurate method for counting ac id-fast hacteria ( AFB) is essential for much work with Mycobacterium leprae. The procedure used , previously in the laboratory (~, 4 ) had two technical difficulties: (a) In order for the drops to spread satisfactorily the microscope slides had to have a uniform wetability of the correct degree; sometimes this requirement was difficul...

Counting small RNA in pathogenic bacteria.

Here, we present a modification to single-molecule fluorescence in situ hybridization that enables quantitative detection and analysis of small RNA (sRNA) expressed in bacteria. We show that short (~200 nucleotide) nucleic acid targets can be detected when the background of unbound singly dye-labeled DNA oligomers is reduced through hybridization with a set of complementary DNA oligomers labele...

Counting and Imaging Bacteria on Mineral Surfaces

Direct Counting 45 Procedure 1: DAPI Cell Count: Sandy Sediment 46 Rock MPN 47 SEM Surface Counts 48 Quantitative FISH 48 Procedure 2: Sample Collection and Preservation for FISH 50 Procedure 3: Slide Preparation and Cell Fixation for FISH 50 Procedure 4: Hybridization 51 Procedure 5: Post-hybridization 52 Procedure 6: Examination and Quantification 53 Chemical Biomass 53 Procedure 7: Lipid Pho...

New method for counting bacteria associated with coral mucus.

The ability to count bacteria associated with reef-building corals in a rapid, reliable, and cost-effective manner has been hindered by the viscous and highly autofluorescent nature of the coral mucus layer (CML) in which they live. We present a new method that disperses bacterial cells by trypsinization prior to 4',6-diamidino-2-phenylindole (DAPI) staining and quantification by epifluorescenc...